Science – Future of Lithuania / Mokslas – Lietuvos Ateitis, Vol 2, No 1 (2010)

Automatic Selection of Two-Dimensional Electrophoresis Gel with Least Geometric Distortions

Dalius Matuzevičius


The paper presents an algorithm for automated selection of the highest quality two-dimensional gel electrophoresis image. The quality criterion is the amount of vertical geometric distortions of the gel. The aim is to select the least distorted gel from the group received during the same biochemical experiment. Vertical geometric distortions displace proteins of the same molecular mass from the horizontal line and have a greater impact on the determination of protein characteristics than horizontal distortions. After presenting algorithm for evaluation of distortions and selection of base gel results are compared to expert's made selections. If necessary, algorithm may be adapted for horizontal distortion evaluation.

Article in Lithuanian

Article in: Lithuanian

Article published:

Keyword(s): two-dimensional electrophoresis; 2DE; geometric distortions; automatic gel analysis

DOI: 10.3846/mla.2010.002

Full Text: PDF pdf

Science – Future of Lithuania / Mokslas – Lietuvos Ateitis ISSN 2029-2341, eISSN 2029-2252
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 License.